ZHOU Quan, TANG Xingguo, HUANG Zhenxing, SONG Pengfei, ZHOU JunjiangBioengineering Department, Wuhan Bioengineering Institute, Wuhan, Hubei , China
Production of Agaricus blazei(strain ABM-3) spawn on four different substrates was compared.A substrate consisting of(w/w) 78% peat,10% cotton seed hull,10% wheat bran,1% CaSO4 and 1% sucrose exhibited superior characteristics in terms of a shorter incubation time required to produce the spawn,a longer spawn shelf-life,and lower costs compared with traditional wheat grain substrate.A novel method for cultivating A.blazei fruit bodies was tested using four different compost formulae.Each compost type was fermented for one week,transferred to polyethylene bags,sterilized at 121 ℃ for 2.5 h,inoculated and incubated until the fungal mycelium had fully colonized the bags.Ten bags were packed into a standard container(57 cm×36 cm×18 cm),cased with peat and incubated until the onset of fruiting.Using this procedure,fresh mushroom yields of 9.0 kg/m2 were achieved with compost consisting of 100 kg cotton seed hulls,10 kg rice hulls,10 kg cow manure,5 kg wheat bran,1 kg CaCO3 and 1 kg CaSO4.These yields are comparable to those obtained using the traditional bed cultivation method.Cadmium levels of 0.42 mg/kg fresh weight were detected in the fruit bodies,which are in accord with national quality standards.
