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食用菌学报 2017, Vol. 24 Issue (02) :88-92    DOI: 10.16488/j.cnki.1005-9873.2017.02.20
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八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响
于荣利,贾 薇,张劲松,杨 焱,吴 迪,唐传红,颜梦秋,庄海宁,汪雯翰*
上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心, 国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403
Effect of Ethanolic Extracts from Eight Kinds of Medicinal and Edible Fungi on Breast Cancer Cell MCF-7 in vitro
YU Rongli,JIA Wei,ZHANG Jingsong,YANG Yan,WU Di,TANG Chuanhong, YAN Mengqiu,ZHUANG Haining,WANG Wenhan?
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences;Key Laboratory of Edible Fungi Resources and Utilization(South), Ministry of Agriculture,P.R.China;National Engineering Research Center of Edible Fungi;National R&D Center for Edible Fungi Processing;Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403,China
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摘要 

以乳腺癌细胞株MCF-7为实验对象,采用细胞增殖抑制率、细胞粘附抑制率、细胞克隆数和细胞迁移距离为评价指标,研究8种食药用菌醇提物对乳腺癌的影响.刺芹侧耳(Pleurotus eryngii)、姬松茸(Agaricus blazei)和灰树花(Grifola frondosa)的醇提物(200 μg/mL)处理能显著降低MCF-7细胞的克隆形成数和细胞迁移距离,较阴性对照分别降低了64.3%、64.5%、61.6%和54.8%、54.8%、48.0%,3种醇提物对MCF-7细胞粘附数和细胞增殖的抑制率分别为48.5%、55.1%、49.3%和30.0%、34.1%、29.5%;另外5种食用菌醇提物作用效果不明显.结果初步表明,刺芹侧耳、姬松茸和灰树花的醇提物(200 μg/mL)具有一定的体外抑制乳腺癌活性.

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作者相关文章
于荣利
贾薇
张劲松
杨焱
吴迪
唐传红
颜梦秋
庄海宁
汪雯翰
关键词MCF-7细胞   细胞增殖   粘附能力   细胞迁移   细胞克隆     
Abstract

Effect of ethanolic extracts from medicinal and edible fungi (eight kinds) was evaluated on the breast cancer cell line, MCF-7, by detecting number of cell colon, inhibition rate of cell adherence, cell migration distance and inhibition rate of cell proliferationcell.Experimental data showed that pretreatments with 200 μg/mL Pleurotus eryngii, Agaricus blazei and Grifola frondosa extracts could significantly reduce clonal formation by 64.3%, 64.5%, 61.6% and cell migration distance by 54.8%,54.8%,48.0%, respectively, compared with negative control.Their inhibitory effects on the adhesion and cell proliferation were at the rate of 48.5%,55.1%,49.3% and 30.0%,34.1%,29.5%, respectively.But no significant effect on the four parameters of cells was recorded for other five tested extracts compared with negative control.Our data revealed that among 8 kinds of fungi evaluated in this study, only P.citrinopileus, A.blazei and G.frondosa showed anti-breast cancer activity in vitro.

KeywordsMCF-7cells,   cell proliferation,   adhesion,   cel lmigration,   cell cloning     
收稿日期: 2016-12-19;
基金资助:

上海市农业科学院青年人才成长计划[沪农青字(2016)第1-14号]和上海市农业科学院科技发展基金 [农科发2012(08)]资助

通讯作者 汪雯翰     Email: wangwenhan@saas.sh.cn
作者简介: 于荣利(1976-),女,硕士,副研究员,主要从事食药用菌研究和编辑工作
引用本文:   
于荣利, 贾薇, 张劲松等 .八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响[J]  食用菌学报, 2017,V24(02): 88-92
YU Rongli, JIA Wei, ZHANG Jingsong etc .Effect of Ethanolic Extracts from Eight Kinds of Medicinal and Edible Fungi on Breast Cancer Cell MCF-7 in vitro[J]  , 2017,V24(02): 88-92
链接本文:  
http://172.17.100.9/CN/10.16488/j.cnki.1005-9873.2017.02.20      或     http://172.17.100.9/CN/Y2017/V24/I02/88
 
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