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食用菌学报 2017, Vol. 24 Issue (02) :75-78    DOI: 10.16488/j.cnki.1005-9873.2017.02.17
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林芝松口蘑抗肿瘤组分的筛选
刘振东1,汪雯翰2,李梁1,薛蓓1*,岳筱1,王绅1
1西藏农牧学院食品科学学院,西藏林芝860000;2上海市农业科学院食用菌研究所, 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心, 国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验,上海 201403
Inhibition of Tumor-Cell Proliferation by Extracts of Tricholoma matsutake Fruit Bodies from Linzhi, Tibet
LIU Zhendong1,WANG Wenhan2,LILiang1,XUEBei1*,YUEXiao1,WANG Shen1
1Xi Zang Agriculture and Animal Husbandry College,Linzhi,Tibet 860000;2Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences;Key Laboratory of Edible Fungi Resources and Utilization(South), Ministry of Agriculture,P.R.China;National Engineering Research Center of Edible Fungi;National R&D Center for Edible Fungi Processing;Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403,China
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摘要 

为筛选林芝松口蘑(Tricholoma matsutake)子实体中具抗肿瘤作用的组分,采用MTT法测定林芝松口蘑乙醇提取物的不同有机溶剂萃取分部对乳腺癌细胞(MCF-7)、胃癌细胞(SGC-7901)和肺癌细胞(A549)的体外抑制率.结果表明:林芝松口蘑乙醇提取物的抑癌活性物质主要集中在氯仿分部,100 μg/mL作用48 h,对MCF-7和SGC-7901的抑制率分别达到了64%和55%;乙醇提取物的各萃取分部对A549抑制效果较差,在实验浓度范围内抑制率均未超过25%.

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刘振东
汪雯翰
李梁
薛蓓
岳筱
王绅
关键词肺癌细胞(A549)   乳腺癌细胞(MCF-7)   胃癌细胞(SGC-7901)     
Abstract

Tricholoma matsutake fruit bodies from Linzhi, Tibet (2 kg) were suspended in 15 volumes of 95% ethanol and extracted under reflux at 85 ℃ for 2 h.After filtration, the residue was twice more subjected to the same procedure and the combined extracts were evaporated to dryness.An aliquot (20 g) of this ethanolic extract was then sequentially extracted with 100 mL of petroleum ether, chloroform, ethyl acetate, n-butanol and double-distilled water, and the extracts evaporated to dryness in each case.Extracts were then tested for their capacity to inhibit the proliferation of the cancer cell lines A549 (lung), MCF-7 (breast) and SGC-7901 (gastric) using the MTT assay.Anti-tumor components present in the T.matsutake fruit bodies were located mainly in chloroform extracts, with MCF-7 and SGC-7901 inhibition rates of 64% and 55%, respectively recorded after 48 h exposure.Proliferation of A549 cells was also inhibited by all the extracts although inhibition rates never exceeded 25% over the range of concentrations adopted.

KeywordsLung cancer cells (A549),   breast cancer cells (MCF-7),   gastric cancer cells (SGC-7901)      
收稿日期: 2016-12-14;
基金资助:

西藏自治区厅校联合基金重点项目(2016ZR-NZ-03)资助

通讯作者 薛蓓     Email: 13658940092@163.com
作者简介: 刘振东(1986-),男,硕士,讲师,主要研究方向为微生物资源与天然产物开发与利用
引用本文:   
刘振东, 汪雯翰, 李梁等 .林芝松口蘑抗肿瘤组分的筛选[J]  食用菌学报, 2017,V24(02): 75-78
LIU Zhendong, WANG Wenhan, LILiang etc .Inhibition of Tumor-Cell Proliferation by Extracts of Tricholoma matsutake Fruit Bodies from Linzhi, Tibet[J]  , 2017,V24(02): 75-78
链接本文:  
http://172.17.100.9/CN/10.16488/j.cnki.1005-9873.2017.02.17      或     http://172.17.100.9/CN/Y2017/V24/I02/75
 
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