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食用菌学报 2017, Vol. 24 Issue (02) :59-62    DOI: 10.16488/j.cnki.1005-9873.2017.02.13
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蛹虫草中游离糖醇、小分子糖类成分研究
朱丽娜1,2,周帅2,刘艳芳2,张忠2,高新华3,唐庆九2?
1上海交通大学农业与生物学院,上海 200240;2上海市农业科学院食用菌研究所, 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心, 上海市农业遗传育种重点开放实验,上海 201403;3上海市农业科学院生态环境保护研究所,上海 201403
Free Alditol and Low Molecular Sugar Levels in Cordyceps militaris
ZHU Lina1,2,ZHOUShuai2,LIU Yanfang2,ZHANG Zhong2,GAO Xinhua3,TANG Qingjiu2?
1School of Agricultural and Biology,Shanghai Jiaotong University,Shanghai 200240,China; 2Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;National Engineering Research Center of Edible Fungi;Key Laboratory of Resources and Utilization of Edible Fungi(South),Ministry of Agriculture,Shanghai 201403,China;3Institute of ECO-Environment and Plant Protection,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China
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摘要 

建立高效阴离子色谱-脉冲安培法同时测定赤藓糖醇、阿糖醇、海藻糖、甘露醇和葡萄糖的方法,方法学考察结果表明该方法精密度(1.4%~4.4%)、稳定性(2.1%~4.9%)、重复性(0.6%~4.5%)和回收率(95.2%~105.7%)良好.用该方法对13个蛹虫草(Cordyceps militaris)子实体和4个菌丝体样品中游离糖醇、小分子糖类成分进行分析,结果表明蛹虫草子实体中含海藻糖(1.04%~19.17%)、甘露醇(0.96%~3.93%)和葡萄糖(1.46%~14.93%),其中海藻糖和葡萄糖含量呈显著负相关;菌丝体中含甘露醇(3.66%~6.70%)和葡萄糖(0.42%~1.06%)以及少量阿糖醇(0.02%~0.12%).

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朱丽娜
周 帅
刘艳芳
张忠
高新华
唐庆九
关键词甘露糖   海藻糖   葡萄糖   阿糖醇     
Abstract

An accurate and reliable method for the simultaneous determination of erythritol, arabitol, mannitol, trehalose and glucose is described.Relative Standard Deviation (RSD) values for precision, stability, reproducibility and recovery ranged between 1.4%-4.4%, 2.1%-4.9%, 0.6%-4.5% and 84.9%-108.3%, respectively.Free alditol and low molecular sugar levels in 13 samples of Cordyceps militaris fruit bodies determined using this method ranged between 1.04%-19.17% (trehalose), 0.96%-3.93%(mannitol) and 1.46%-14.93% (glucose) dry weight,and there was a significant negative correlation (-0.83) between trehalose and glucose content.Mannitol,glucose and arabitol levels in four samples of C.militaris mycelia grown in submerged culture ranged between 3.66%-6.70%, 0.42%-1.06% and 0.02%-0.12% dry weight, respectively.

KeywordsMannitol,   trehalose,   glucose,   arabitol     
收稿日期: 2017-01-10;
基金资助:

公益性行业(农业)科研专项(201303080)资助

通讯作者 唐庆九     Email: tangqingjiu@saas.sh.cn
作者简介: 朱丽娜(1980-),女,副研究员,主要从事食药用菌活性物质方面研究和《食用菌学报》编辑工作
引用本文:   
朱丽娜, 周 帅, 刘艳芳等 .蛹虫草中游离糖醇、小分子糖类成分研究[J]  食用菌学报, 2017,V24(02): 59-62
ZHU Lina, ZHOU Shuai, LIU Yanfang etc .Free Alditol and Low Molecular Sugar Levels in Cordyceps militaris[J]  , 2017,V24(02): 59-62
链接本文:  
http://172.17.100.9/CN/10.16488/j.cnki.1005-9873.2017.02.13      或     http://172.17.100.9/CN/Y2017/V24/I02/59
 
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