食用菌学报
 首页  |  期刊介绍  |  编 委 会  |  期刊订阅  |  投稿指南  |  广告服务  |  投稿须知  |  联系我们  |  English
食用菌学报 2017, Vol. 24 Issue (02) :38-42    DOI: 10.16488/j.cnki.1005-9873.2017.02.09
研究论文 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << | >>
液相色谱-串联质谱法测定糙皮侧耳中9种植物生长调节剂的残留量
司文帅1,张 颖2,聂冬霞1,董茂锋1,宋卫国1,白 冰1?
1上海市农业科学院农产品质量标准与检测技术研究所,上海 201403;2上海健康医学院,上海 201318
Determination of Nine Plant Growth Regulator Residues in Pleurotus ostreatus Fruit Bodies Using LC-MS/MS
SI Wenshuai1,ZHANG Ying2,NIE Dongxia1,DONG Maofeng1,SONG Weiguo1,BAI Bing1
1Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences, Shanghai 201403,China;2Shanghai University of Medicine & Health Sciences,Shanghai 201318,China
Download: PDF (0KB)   HTML (1KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
摘要 

建立了液相色谱-串联质谱仪(Liquid Chromatography-Tandem Mass Spectrometry,LC-MS/MS)同时测定糙皮侧耳(Pleurotus ostreatus)中吲哚乙酸、吲哚丙酸、吲哚丁酸、赤霉素、2,4-二氯苯氧乙酸、6-苄基腺嘌呤、4-氯苯氧乙酸、6-糠氨基嘌呤和芸苔素内酯9种植物生长调节剂残留的定量分析方法,样品经含1%(v/v)乙酸的乙腈溶液提取,于分散固相萃取管(含混匀的150 mg MgSO4和50 mg C18)净化后用LC-MS/MS检测.结果表明:9种植物生长调节剂的峰面积与其浓度呈良好线性关系,方法最低检出限为0.5~1.0 μg/kg;回收率为80.3%~96.5%,相对标准偏差为0.88%~7.31%,该方法准确、可靠,可以同时满足糙皮侧耳中9种植物生长调节剂残留检测的需要.对市场上50份糙皮侧耳的测定结果为:吲哚乙酸检出6次(2.3~96.7 μg/kg)、吲哚丙酸2次(21.3、87.8 μg/kg)、吲哚丁酸5次(3.4~91.2 μg/kg)

Service
把本文推荐给朋友
加入我的书架
加入引用管理器
Email Alert
RSS
作者相关文章
司文帅
张颖
聂冬霞
董茂锋
宋卫国
白冰
关键词糙皮侧耳   基质分散固相萃取   植物生长调节剂     
Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to simultaneously determine the residual levels of nine plant growth regulators (3-indole-acetic acid, indole-3-propionic acid, 3-indolebutyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, 4-chlorophenoxyacetic acid, kinetin and brassinolide) in Pleurotus ostreatus fruit bodies.Samples were first extracted with acetonitrile containing 1% (v/v) acetic acid, clarified by dispersive solid-phase extraction using a mixture of MgSO4 (150 mg) and C18(50 mg), and analyzed by LC-MS/MS.Standardization experiments revealed the methodology to be accurate and reliable, with calibration curves exhibiting good linearity over the concentration range 0.5-200 μg/L and detection limits of 0.5-1.0 μg/kg.Recovery rates ranged between 80.3% and 96.5% with Relative Standard Deviation values varying between 0.88% and 7.31%.Of 50 P.ostreatus fruit body samples obtained from supermarkets and farm product markets, 3-indole-acetic acid (2.3-96.7 μg/kg) was detected on six occasions, and indole-3-propionic acid (21.3 and 87.8 μg/kg) and 3-indolebutyric acid (3.4-91.2 μg/kg) each five times.

KeywordsPleurotus ostreatus,   dispersive solid-phase extraction,   plant growth regulator     
收稿日期: 2016-11-09;
基金资助:

上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-25号]资助

通讯作者 白冰     Email: qs11@saas.sh.cn
作者简介: 司文帅(1987-),男,硕士,主要从事农产品检测技术研究
引用本文:   
司文帅, 张颖, 聂冬霞等 .液相色谱-串联质谱法测定糙皮侧耳中9种植物生长调节剂的残留量[J]  食用菌学报, 2017,V24(02): 38-42
SI Wenshuai, ZHANG Ying, NIE Dongxia etc .Determination of Nine Plant Growth Regulator Residues in Pleurotus ostreatus Fruit Bodies Using LC-MS/MS[J]  , 2017,V24(02): 38-42
链接本文:  
http://172.17.100.9/CN/10.16488/j.cnki.1005-9873.2017.02.09      或     http://172.17.100.9/CN/Y2017/V24/I02/38
 
没有本文参考文献
[1] Andrej GREGORI,Bojan PAHOR,Franc POHLEVEN,.迷迭香(Rosmarinus officinalis)提取残渣栽培糙皮侧耳(英文)[J]. 食用菌学报, 2008,15(02): 18-22
[2] 陈世昌,宋志伟,王小琳,徐明辉.复合微生物制剂对糙皮侧耳菌丝生长和子实体产量的影响[J]. 食用菌学报, 2005,12(02): 55-59
[3] 黄春燕,单洪涛,刘传富,皇传华,万鲁长,郭惠东,杨化恩,张福进.糙皮侧耳液体菌种培养条件的初步研究[J]. 食用菌学报, 2004,11(04): 44-50
[4] 杜爱玲,侯军,王林生,吴祖峰.甘薯渣在食用菌制种及栽培上的应用[J]. 食用菌学报, 2003,10(03): 36-40
[5] 王淑珍,范俊,扬家峰,扬英杰,白晨.灵芝与糙皮侧耳原生质体融合子生物学特性的初步研究[J]. 食用菌学报, 2003,10(02): 5-8
[6] 王淑珍,白晨,范俊,高雁,扬家峰,扬英杰.灵芝与糙皮侧耳原生质体融合子基因组RAPD分析[J]. 食用菌学报, 2003,10(01): 1-5
[7] 蒋冬花.四种杀菌剂对糙皮侧耳菌丝生长和子实体产量的影响及控制霉菌污染的效果[J]. 食用菌学报, 2002,09(03): 46-51
[8] 万鲁长,黄春燕,曹德强,宫志远,曲玲,程业武.糙皮侧耳褐腐病和褐斑病致病机理研究初报[J]. 食用菌学报, 2001,08(02): 34-38
[9] 王淑芳,卜庆梅,蔡德华.棉籽壳发酵料栽培糙皮侧耳优良菌株的选育[J]. 食用菌学报, 2001,08(01): 30-33
[10] 齐志广,王立安,赵宝华.糙皮侧耳不同栽培料比较试验[J]. 食用菌学报, 2000,07(01): 47-50
[11] 齐志广,王立安,赵宝华.糙皮侧耳在玉米秸秆中的生长发育规律[J]. 食用菌学报, 1999,06(03): 34-38
[12] 赵萍.钾肥对糙皮侧耳产量和品质的影响[J]. 食用菌学报, 1998,05(04): 44-49
[13] 杨国良,李丽娜,陆师义,艾福彪,杨秀琴.侧耳纤维二糖水解酶基因的克隆与表达研究[J]. 食用菌学报, 1998,05(01): 3-7
[14] 郁建强,殷戎一,黄丹枫,蔡云霞,庄天明.麦秸生料栽培糙皮侧耳试验[J]. 食用菌学报, 1998,05(01): 47-53
Copyright by 食用菌学报